Isolation and Characterization Probiotic Bacteria from Curd samples for Evaluation of antagonistic and free radical scavenging activity as a therapeutic approach

• Pranav Suresh Tambe,  
• Dr Gajanan Vishnu Mali,*  
• Ranjana Shirish Chavan

Abstract

Aim: Screening of potential probiotic organisms from different curd samples to evaluate its antagonistic and free radical scavenging activity for therapeutic approach. Background: Probiotic microorganisms play a significant role in promoting gut health. Identifying and characterizing these organisms is very crucial for developing new paths for therapeutic research. Materials and Methods: A total of 40 organisms were isolated from different curd samples using MRS agar under microaerophilic conditions. Based on Gram staining, motility, and endospore formation, 13 isolates were selected for further analysis. Catalase production was assessed in 7 non-endospore forming isolates, with 4 (LC8, 20, 27, and 30) found to be catalase negative. The probable genus of these isolates was determined using Bergey’s Manual of Systematic Bacteriology. Results: The isolates LC8, 20, 27, and 30, which were catalase-negative and non-endospore forming, likely belong to the genus Lactobacillus. Further screening revealed that these isolates were tolerant to a wide range of pH levels, bile tolerant, non-hemolytic, and sensitive to antibiotics. Along with these properties free radical scavenging activity, hydrophobicity testing antagonistic properties were evaluated.16S rRNA sequencing identified the isolates as belonging to Enterococcus faecium and Lentilactobacillus parabuchneri Conclusion: The isolated organisms, identified as Enterococcus faecium and Lentilactobacillus parabuchneri exhibit potential antagonistic and free radical scavenging activities which makes them potent candidates for further therapeutic approach.

Keywords

Probiotics, Lactic acid bacteria, Lactobacillus,16S r RNA, Enterococcus