Detection of inducible clindamycin resistance in staphylococcus aureus and CONS at tertiary care hospital

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Original Article

Author Details : Mallikarjun Koppad, Parameshwar S, Halesh LH, Siddesh KC

Volume : 2, Issue : 4, Year : 2015

Article Page : 192-197

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Abstract

Background: The increasing prevalence of Methicillin resistant among staphylococci (MRSA) is an increasing problem. Increasing incidence of infections due to MRSA has led to emphasis on the need for safe & effective agents to treat both systemic & localized Staphylococcal infections. Clindamycin has been used to treat pneumonia & soft tissue and musculoskeletal infections due to MRSA. One important issue in Clindamycin treatment is the risk of clinical failure during therapy caused by MLSB inducible resistance.
Objectives: To isolate and identify Staphylococcus aureus and CONS from all clinical samples & to determine the inducible Clindamycin resistance among the Staphylococcus aureus and CONS.
Methods: A total of 100 isolates of Staphylococcus aureus and CONS from various samples were isolated. Methicillin resistance was detected by using a 1 ?g Oxacillin disc. The D-test was performed using the discs of Clindamycin (CL)(2?g) and Erythromycin (ER)(15?g) placed at a distance of 15mm (centre to centre) along with routine antibiotic susceptibility testing.
Results: Among Staphylococcus aureus, MSSA isolates were 32(47.05%) compared to MRSA isolates, 26(38.24%) and among CNS, MSCONS isolates were 8(11.77%) compared to MRCONS 2(2.9%).A total of 12(17.64 %) isolates showed iMLSB, of which 8(11.77%) were MRSA, 2(2.9%) were MSSA and 2(2.9%) MRCONS isolates.
Conclusion: Prevalence of inducible Clindamycin resistance among Staphylococcal isolates was significant. Hence the implementation of this D-test routinely, which is simple, reliable & inexpensive will reveals the iMLSB & cMLSB phenotype & prevents the therapeutic failure of Clindamycin.

Keywords: Methicillin resistant Staphylococcus aureus (MRSA), Inducible Clindamycin resistance, D-test, Erythromycin, iMLSB phenotype

How to cite : Koppad M, Parameshwar S, Halesh Lh, Siddesh Kc, Detection of inducible clindamycin resistance in staphylococcus aureus and CONS at tertiary care hospital. Indian J Microbiol Res 2015;2(4):192-197

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