Detection of bioflim production among Staphylococcus.aureus by Congo red method and tube method

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Original Article

Author Details : Sharlee R*, Sumangala B

Volume : 7, Issue : 3, Year : 2020

Article Page : 222-225

https://doi.org/10.18231/j.ijmr.2020.040

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Abstract

Background: Bioflim is a mode of survival for various microbe by which they form aggregates during
unfavourable conditions. Staphylococcus aureus is a major cause of nosocomial and community acquired
infections.
Materials and Methods: A total of 150 Staphylococcus isolates were screened for biofilm production by
Congored method and Tube method following standard guidelines.
Results: Of the 150 isolates, 85(56.6%) were MSSA and 65(43.4%) were MRSA. On Congored agar, 63
isolates showed black colonies with dry crystalline consistency indicating biofilm production. Out of 63
isolates 62% of isolates were MRSA and 38% of isolates were MSSA. Biofilm by tube method, 84isolates
showed biofilm production. MSSA were 48.2% and MRSA were 81.53%.
Conclusion: MRSA is the significant biofilm producer when compare to MSSA, Congored method is less
accurate when compare to tube method as screening test for the detection of biofilm.

Keywords: MRSA-Methicillin resistant Staphylococcus aureus, MSSA- Methicillin sensitive Staphylococcus aureus, Biofilm, Congored.
 

 

How to cite : Sharlee R, Sumangala B, Detection of bioflim production among Staphylococcus.aureus by Congo red method and tube method. Indian J Microbiol Res 2020;7(3):222-225

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