Identification and antibiotic susceptibility pattern of urinary isolates in out-patients at a tertiary care center in a rural set up

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Original Article

Author Details : Yuga B Pawar*, Ujjwala Dehankar

Volume : 7, Issue : 3, Year : 2020

Article Page : 237-241

https://doi.org/10.18231/j.ijmr.2020.043

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Abstract

Background: Urinary tract infection (UTI) is most common infections described in out- patients settings.
In almost all cases empirical antimicrobial treatment initiates before the laboratory results of urine culture
are available, thus antibiotic resistance may increase in uropathogens due to frequent use of antibiotics.
Objective: To identify and to determine antibiotic susceptibility pattern of bacterial pathogens of urinary
tract infection reporting at a tertiary care hospital in OPD only.
Study Design: Cross-sectional.
Place and Duration of Study: Department of microbiology, NKP salve institute of medical science, Lata
Mangeshkar hospital, Nagpur from July to September 2018.
Methodology: A total of 33 culture positive bacterial isolates from 300 urine samples, submitted over a
period of 2 months were included in this study. Identification of bacterial isolates was done by standard
biochemical profile of the organism. The antibiotic susceptibility of culture positive bacterial isolates was
performed by disk diffusion method as recommended by CLSI.
Result: Out of 33 culture positive samples the most prevalent bacterial isolate was E. Coli(48%) followed
by klebsiella(18%), staphylococcus coagulase negative(12%). The susceptibility pattern of E. Coli showed
that 81% of bacterial isolates were sensitive to Nitrofurantoin,75% to CIS and 50% to Gentamicin.
Klebsiella was most sensitive to Gentamicin and CIS. Staphylococcus coagulase negative was most
sensitive to Cd and Vancomycin.
Conclusion: Majority of bacterial isolates were sensitive to nitrofurantoin, CIS, Gentamicin. Among the
oral antibiotics, nitrofurantoin showed good susceptibility against Enterobacteriaceae family and gram
positive organisms.

Keywords: Urinary tract infection, Frequency, Antibiotic susceptibility Urinary, Pathogens.

How to cite : Pawar Y B , Dehankar U, Identification and antibiotic susceptibility pattern of urinary isolates in out-patients at a tertiary care center in a rural set up. Indian J Microbiol Res 2020;7(3):237-241

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