A study on susceptibility patterns, resistance mechanisms and cross- resistances of antibiotics against Pseudomonas aeruginosa in a teaching hospital at Puducherry

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Original Article

Author Details : Hemalatha Gurumurthy*, G K Poongothai, K Bhaskaran

Volume : 8, Issue : 4, Year : 2021

Article Page : 279-284

https://doi.org/10.18231/j.ijmr.2021.057

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Abstract

Background: Pseudomonas aeruginosa, a gram negative bacteria causes lung and nosocomial infections, mostly infect the body after surgery or invasive techniques. There has been a increasing prevalence in drug resistant variants in the recent years.
Objectives: 1. To determine the antibiotic susceptibility patterns of P.Aeruginosa; 2. To assess the antibiotics used against P. aeruginosa and the cross resistance pattern existing between them; 3. To evaluate the possible resistance mechanisms of P.aeroginosa by phenotypic techniques.
Materials and Methods: Thirty-six consecutive, nonduplicate P. aeruginosa isolates were collected between January to July in the year 2018 from the hospital pus samples. The isolates showed synthesis of pyocyanin and a oxidase positive reaction. Kirby bauer’s disc diffusion method (HIMEDIA). was used for assessing the sensitivity of drugs. Disk approximation test was done to check the prevalence of inducible lactamases. Modified Hodge test was done to assess the metallo-lactamase activity. Double disk synergy method had been preffered to evaluate the extended-spectrum beta-lactamase (ESBL) activity.
Results: The most sensitive antibiotic was found to be ciprofloxacin which is followed by amikacin and ceftazidime (p < 0> Conclusion: Strict adherence to the recent trend of “reserve drugs” concept and minimizing the misuse of antibiotics can bring down the drug resistance and morbidity. The addressal of irrational and inappropriate use of antimicrobials among the clinician is the need of the hour.

Keywords: Pseudomonas aeuroginosa, Sensitivity, Reisitance.

How to cite : Gurumurthy H, Poongothai G K, Bhaskaran K, A study on susceptibility patterns, resistance mechanisms and cross- resistances of antibiotics against Pseudomonas aeruginosa in a teaching hospital at Puducherry. Indian J Microbiol Res 2021;8(4):279-284

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