A pilot study on comparative analysis of minimum inhibitory concentration and mutant prevention concentration of conjunctival bacterial isolates against fluoroquinolones

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Original Article

Author Details : Krishnan Selvi, A. V. Kavitha, Sunderesan Anand, Durgadevi Parthasarathy, Lakshmipathy Meena*

Volume : 11, Issue : 3, Year : 2024

Article Page : 180-185

https://doi.org/10.18231/j.ijmr.2024.033

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Abstract

Background: The aim of this study was to compare the Minimum Inhibitory Concentration (MIC) and Mutant Prevention Concentration (MPC) of fluoroquinolones, including ciprofloxacin, moxifloxacin, and gatifloxacin, against Staphylococcus aureus and coagulase-negative Staphylococci (CONS) isolated from conjunctival swabs.
Materials and Methods: 25 isolates of Staphylococcus spp., obtained from conjunctival swabs submitted to the Department of Microbiology, Vision Research Foundation, Sankara Nethralaya, were included in this study. The identification and confirmation of Staphylococcus spp. were performed using standard microbiological techniques. The MIC and MPC were determined using the agar dilution method, following protocols from previous studies. The MIC, MIC, MPC, and MPC values for the above three fluoroquinolones were calculated and analysed.
Results: Out of all 25 isolates, 20 were CONS and 5 were Staphylococcus aureus. In our study, gatifloxacin had least MIC and MPC values when compared to ciprofloxacin and moxifloxacin of gatifloxacin had lower MPCand MPC values in comparison to ciprofloxacin and moxifloxacin. Our study shows that Gatifloxacin had least MIC and MPC values when compared to ciprofloxacin and moxifloxacin. Besides, MPC of ciprofloxacin, moxifloxacin and gatifloxacin showed wider range of distribution than the MIC.
Conclusion: Gatifloxacin demonstrated effective inhibition of resistant mutant strains at lower concentrations compared to ciprofloxacin and moxifloxacin. Additionally, future studies with a larger number of isolates, incorporating pharmacokinetic and pharmacodynamic parameters, will provide essential information on therapeutic outcomes and resistance prevention.
 

Keywords: Staphylococcus aureus, Minimal inhibitory concentration, Mutant prevention concentration, Fluoroquinolones.

How to cite : Selvi K, Kavitha A V, Anand S, Parthasarathy D, Meena L, A pilot study on comparative analysis of minimum inhibitory concentration and mutant prevention concentration of conjunctival bacterial isolates against fluoroquinolones. Indian J Microbiol Res 2024;11(3):180-185

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