Comparative evaluation of broth microdilution, E-strip and Vitek-2 for colistin susceptibility among carbapenem resistant Acinetobacter Spp and Klebsiella Spp


Original Article

Author Details : Monisha Basavaraju, M Raghavendra Rao*, Ranjitha Shankaregowda, Rashmi P Mahale, Sowmya G Shivappa, Vidyavathi B Chitharagi

Volume : 11, Issue : 4, Year : 2024

Article Page : 243-247

https://doi.org/10.18231/j.ijmr.2024.043



Suggest article by email

Get Permission

Abstract

Background: Gram-negative bacterial infections that are resistant to carbapenems are a serious clinical problem. The only effective drugs against them are still tigecycline and colistin. In fact, polymyxin E, also known as colistin, has been regarded as a "last resort" antibiotic. But in recent years, colistin resistance has increased globally, significantly reducing treatment options and highlighting the significance of accurate colistin testing methods to support appropriate therapeutic decision-making.
Materials and Methods: The primary goal of the study is to compare the effectiveness of E-test and Vitek 2 for colistin sensitivity testing to the conventional microbroth dilution method using 120 clinical isolates of Klebsiella pnuemoniae and Acinetobacter baumannii that are resistant to carbapenem over the course of a year, isolated from January to December 2019.
Results: In comparison to Vitek-2, the results indicated that brothmicro dilution and E-test produced the narrowest range of colistin MICs. After comparing the E-test’s errors and overall agreement with BMD, 100% of the Essential Agreement, 94.27% of the Categorical Agreement, and 5.72% of the Very Major Errors with no Major Errors were found. Comparing Vitek-2 with BMD, similar results were found: 1.66% MEs, 98.32% CAs, 80.99% EAs, and no VMEs.
Conclusion: As a result, it was determined that, in contrast to Vitek-2, MBD and E-test had distinct MICs, making them appropriate for determining colistin MIC. It will take more research on automated systems to standardize colistin susceptibility testing procedures, particularly for and K. pnuemoniae and A. baumannii.

Keywords: Broth microdilution, Carbapenem, Colistin, E- test, Vitek 2, Acinetobacter, Klebsiella.


How to cite : Basavaraju M, Rao M R, Shankaregowda R, Mahale R P, Shivappa S G, Chitharagi V B, Comparative evaluation of broth microdilution, E-strip and Vitek-2 for colistin susceptibility among carbapenem resistant Acinetobacter Spp and Klebsiella Spp. Indian J Microbiol Res 2024;11(4):243-247


This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.







Article History

Received : 20-04-2024

Accepted : 14-10-2024


View Article

PDF File   Full Text Article


Copyright permission

Get article permission for commercial use

Downlaod

PDF File   XML File   ePub File


Digital Object Identifier (DOI)

Article DOI

https://doi.org/ 10.18231/j.ijmr.2024.043


Article Metrics






Article Access statistics

Viewed: 218

PDF Downloaded: 27