Isolation and characterization of probiotic bacteria from curd samples for evaluation of antagonistic and free radical scavenging activity as a therapeutic approach


Original Article

Author Details : Pranav Suresh Tambe, Gajanan Vishnu Mali*, Ranjana Shirish Chavan

Volume : 11, Issue : 4, Year : 2024

Article Page : 269-276

https://doi.org/10.18231/j.ijmr.2024.047



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Abstract

Background: Probiotic microorganisms play a significant role in promoting gut health. Identifying and characterizing these organisms is very crucial for developing new paths for therapeutic research.
Aim: Screening of potential probiotic organisms from different curd samples to evaluate its antagonistic and free radical scavenging activity for therapeutic approach.
Materials and Methods: A total of 40 organisms were isolated from different curd samples using MRS agar under microaerophilic conditions. Based on Gram staining, motility, and endospore formation, 13 isolates were selected for further analysis. Catalase production was assessed in 7 non-endospore forming isolates, with 4 (LC8, 20, 27, and 30) found to be catalase negative. The probable genus of these isolates was determined using Bergey’s Manual of Systematic Bacteriology.
Results: The isolates LC8, 20, 27, and 30, which were catalase-negative and non-endospore forming, likely belong to the genus Lactobacillus. Further screening revealed that these isolates were tolerant to a wide range of pH levels, bile tolerant, non-hemolytic, and sensitive to antibiotics. Along with these properties free radical scavenging activity, hydrophobicity testing antagonistic properties were evaluated.16S rRNA sequencing identified the isolates as belonging to Enterococcus faecium and Lentilactobacillus parabuchneri.
Conclusion: The isolated organisms, identified as Enterococcus faecium and Lentilactobacillus parabuchneri exhibit potential antagonistic and free radical scavenging activities which makes them potent candidates for further therapeutic approach.


Keywords: Probiotics, Lactic acid bacteria, Free radical scavenging, Enterococcus faecium, Lentilactobacillus parabuchneri,  Antagonistic, Curd.


How to cite : Tambe P S, Mali G V, Chavan R S, Isolation and characterization of probiotic bacteria from curd samples for evaluation of antagonistic and free radical scavenging activity as a therapeutic approach. Indian J Microbiol Res 2024;11(4):269-276


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Article History

Received : 22-08-2024

Accepted : 14-10-2024


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https://doi.org/ 10.18231/j.ijmr.2024.047


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