Incidence & Estimation of Beta – Lactamase Enzymes (ESBL Ampc, Carbapenemase Enzymes) singly and their coexistence in clinical isolates of Gram Negative bacteria by Vitek – 2 Compact System

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Original Article

Author Details : Archana Bora, PK Khatri, Aruna Solanki, RS Parihar, AK Chandora

Volume : 3, Issue : 4, Year : 2016

Article Page : 352-358

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Abstract

Introduction: With increase in Beta – Lactamases producing microbial infections there is need for rapid method for identification of different types of Beta Lactamases enzyme production & their coexistence in clinical isolates. Vitek – 2 compact play very crucial role in detection of different types of Beta- Lactamases & their coexistence.
Materials & Methods: 240 clinical specimen like Urine, Tracheal Secretion, Sputum, pus & Stool were isolated from clinical samples of OPD & IPD Patients of Tertiary Care Hospitals attached to Dr. S.N. Medical College, Jodhpur. Identification of Clinical isolates & detection of Betalactamase enzymes with their antibiotic susceptibility pattern was done by automated Vitek – 2 compact System.
Results: 146 (60.83%) E. coli, 67 (27.91%) Klebsiella, 22 (9.17%) Enterobacter, 2 (0.83%) Citrobacter & 3 (1.25%) Serratia spp. were included in present study. Out of 240 isolates 195 (81.25%) were enzymes producers & 45(8.75%) were non-enzyme producer. Of the 195 isolates 147(61.25) were single enzyme producer & 48(20%) isolates were multiple enzyme producers. 105(44%) isolates shows only ESBL enzyme production, 26 (11%) isolates shows AMPc enzyme & 16(7%) isolates shows carbapenemase enzymes production. Of the 48 isolates producing mixed enzymes 45.83% shows ESBL+AMPc and ESBL+CARBAPENEMASE each and 16.66% shows all enzymes (ESBL+AMPc+CARBAPENEMASE) production. Isolates producing ESBL are maximally resistant to Cefepime followed by Cefuroxime, & Ceftriaxone. Organism producing AMP care maximally Resistant to Cefuroxime followed by Trimethoprim - Sulfmethoxazole, Piperacillin-Tazobactum, Ampicillin. Organism producing CARBAPENEMASE are maximally Resistant to Ampicillin, Cefipime followed by Cefuroxime+Sulbactum, and Amoxicillin+Clavulinic acid. Organism producing mixed enzymes are maximally resistant to Cefuroxime, followed by Amoxicillin+Clavulinic acid and Ciprofloxacin.
Conclusion: The study show that Betalactamase producing microbial organism are very common in Hospital environment and responsible for majority of infections. Vitek-2 compact is found to be rapid and reliable method to identify Betalactamase and their co-existence. It is better than conventional method.

Keywords: Beta lactamases, Vitek – 2, E. coli, Klebseilla

How to cite : Bora A, Khatri P, Solanki A, Parihar R, Chandora A, Incidence & Estimation of Beta – Lactamase Enzymes (ESBL Ampc, Carbapenemase Enzymes) singly and their coexistence in clinical isolates of Gram Negative bacteria by Vitek – 2 Compact System. Indian J Microbiol Res 2016;3(4):352-358

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