Resazurin assay for rapid drug susceptibility testing of Mycobacterium tuberculosis

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Original Article

Author Details : Mangala Ghatole, Virendra Kashetty, Apeksh Ghule

Volume : 5, Issue : 1, Year : 2018

Article Page : 138-142

https://doi.org/10.18231/2394-5478.2018.0028

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Abstract

Introduction: Tuberculosis, a disease of antiquity continues to cause high morbidity and mortality. In Sustainable Development Goals and End Tb Strategy proposed by WHO includes Drug Susceptibility Testing as major component. Conventional methods are time consuming and molecular methods are rapid but expensive and demanding. Resazurin assay is a rapid colorimetric redox indicator method of Drug Susceptibility Testing.
Materials and Methods: 67 strains of M. tuberculosis isolated from pulmonary tuberculosis patients were tested using indirect absolute concentration method by resazurin assay and compared with drug incorporated Lowenstein – Jensen media for Isoniazid and Rifamycin.
Results: There was 91.5 % and 91.7% concordance between both methods for INH and Rifamycin respectively. Resazurin assay for INH was 100% sensitive and 61.54% specific. Sensitivity and specificity for RMP was 98.04% and 62.5% respectively. The duration required for INH was 7.0 ± 2.07 and for RMP 6.58 ± 1.58 days.
Conclusions: Resazurin assay is sensitive, rapid, cheap, technically simple drug susceptibility method.

Keywords: M. tuberculosis, resazurin assay, drug susceptibility.

How to cite : Ghatole M, Kashetty V, Ghule A, Resazurin assay for rapid drug susceptibility testing of Mycobacterium tuberculosis. Indian J Microbiol Res 2018;5(1):138-142

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