ISOLATION AND CHARACTERIZATION OF STARCH DEGRADING BACTERIA FROM GARDEN SOIL, GANPAT UNIVERSITY, GUJARAT, INDIA

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Original Article

Author Details : Gayatri Patel

Volume : 2, Issue : 2, Year : 2015

Article Page : 111-114

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Abstract

Background: Enzymes are the most important substances used in so many areas either in research, medicine or commonly in industries. Starch degrading bacteria are mostly important in food, texture, fermentation and paper industries. Amylase production from bacteria is economical because the enzyme production rate is higher in bacteria as compared to other microorganism. The isolation and manipulation of pure culture of starch degrading microorganisms from soil have a great importance on biotechnology as well as microbiology field.

Methods: Present study involves the isolation by dilution method, characterization and identification by bergey’s menual of systematic bacteriology. Enzyme assays by DNS a method. Optimization of enzymes at different parameters.

Results: In this research the bacterial four strains A1, A2, A3 and A4 were isolated from soil at area of college campus Ganpat University, Gujarat, India. Isolated bacteria have great amylase activity at 37?Ctemperature, the highest hydrolysis zone of starch using with concentration of 1.5% amylase activity at different pH in highest growth of bacteria at pH 6.7 and amylase activity at different time duration at 48hrs with high activity 23.5U/ml in isolate A4 by DNS a method.

Conclusions: In the presence study, the bacteria were isolated from the garden soil from the college campus. This area also consisting of starchy materials we found starch degrading bacteria have capacity to grow in physical condition pH, temperature and concentration of starch. As per morphological character, colony character and biochemical testing isolated bacteria similar to genus bacillus.

Key Words: Soil sample, Amylase enzyme, Bacillus spp., Starch degrading, DNS method.

How to cite : Patel G, ISOLATION AND CHARACTERIZATION OF STARCH DEGRADING BACTERIA FROM GARDEN SOIL, GANPAT UNIVERSITY, GUJARAT, INDIA. Indian J Microbiol Res 2015;2(2):111-114

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