PCR as diagnostic tool in early detection of extra pulmonary tuberculosis

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Original Article

Author Details : Manali Shah, Ullas Bhabhor, Yogita Mistry, Summaiya Mullan

Volume : 5, Issue : 2, Year : 2018

Article Page : 171-173

https://doi.org/10.18231/2394-5478.2018.0036

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Abstract

Introduction: Extra-pulmonary tuberculosis is a major public health issue with a significant cause of morbidity and mortality in developing countries. Diagnosis is difficult due to various clinical presentations and pauci-bacillary nature. The less number of tubercular bacteria at extra-pulmonary sites may cause diagnostic difficulty demanding for rapid, sensitive and specific test.  
Material and Methods:It was a 1 year retrospective study in tertiary care hospital. Different extra-pulmonary samples received by laboratory were included in the study. Microscopy by ZN stain, culture on Lowenstein-Jensen media and RT-PCR on ABI 7300 was performed after decontamination of the samples using 4% NaOH and 2.9% sodium citrate.
Results:Total 300 extra-pulmonary samples were tested. 122 samples were positive by either method. Sensitivity of PCR was 59%. ZN microscopy and culture method showed 46% and 48% sensitivity respectively.  Tubercular bacilli were isolated were mainly body fluids, pus and endometrial biopsy. The most common age group affected was 20-29 years.
Conclusion:PCR is still a good diagnostic tool for extra-pulmonary tuberculosis even if CBNAAT is available. Even more comparative studies should be done between different molecular methods, so that in future the best option for diagnosis of extra-pulmonary tuberculosis without much more false negative results will be available.

Keywords: Extra pulmonary, Tuberculosis, PCR

How to cite : Shah M, Bhabhor U, Mistry Y, Mullan S, PCR as diagnostic tool in early detection of extra pulmonary tuberculosis. Indian J Microbiol Res 2018;5(2):171-173

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