Study of lead and nickel resistance mechanism in Enterobacter species

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Original Article

Author Details : Nirbhavane H M, Bagde U S

Volume : 5, Issue : 2, Year : 2018

Article Page : 229-235

https://doi.org/10.18231/2394-5478.2018.0048

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Abstract

The aim of this study was to understand the mechanism of resistance towards the heavy metal Lead and Nickel using resistant and sensitive Enterobacter spp. The cellular biochemistry of this microorganism was checked with the help of estimations of dehydrogenases activity, and at the molecular biology level determination of Lead and Nickel resistant plasmid through Agarose gel Electrophoresis. The Minimum inhibitory concentration (MIC) determined was 100 ppm (Lead) and 50 ppm (Nickel) in sensitive Enterobacter spp. that completely inhibited the growth, and activity of dehydrogenases of the TCA (Tricarboxylic Acid) cycle. The tolerance level of Lead and Nickel in the resistant Enterobacter spp., were found to be 300 ppm and 200 ppm respectively. Mechanism of resistance was found to be the presence of the resistance plasmid.

Keywords: Lead, Nickel, Resistant, Sensitive, Enterobacter, Mechanism

How to cite : Nirbhavane H M, Bagde U S, Study of lead and nickel resistance mechanism in Enterobacter species. Indian J Microbiol Res 2018;5(2):229-235

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