Expression of Assimilatory Nitrate and Nitrite Reductase of Rhizobium meliloti

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Original Article

Author Details : Anup Kumar Halder, P. K. Chakrabartty

Volume : 2, Issue : 3, Year : 2015

Article Page : 133-137

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Abstract

Purpose: To investigate the expression of assimilatory nitrate- and nitrite reductase activity of Rhizobium meliloti SU 47.

Methods: Rhizobium cell were grown in medium, after 20 hrs cells were washes with potassium phosphate buffer and assayed the enzyme activity through nitrite estimation.

Result: Expression of assimilatory nitrate- and nitrite reductase was observed in GTS/glutamate medium. The enzymes were inducible in nature since it expressed only in presence of nitrate or nitrite. Ammonium inhibited the enzyme activity. Cyanate acted as a gratituous inducer of both the enzymes. Molybdenum acts as modulator whereas tungstate as an inhibitor of the enzyme.

Conclusion: Both the enzymes, assimilatory nitrate- and nitrite reductases expressed in GTS/glutamate medium in presence of nitrate.

 

Key Words: Assimilatory nitrate and nitrite reductase, Rhizobium meliloti, Cyanate

How to cite : Halder A K, Chakrabartty P K, Expression of Assimilatory Nitrate and Nitrite Reductase of Rhizobium meliloti. Indian J Microbiol Res 2015;2(3):133-137

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