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- DOI 10.18231/j.ijmr.98133.1758352054
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CrossMark
Efficacy of plant-derived extracts against multidrug resistant pathogens: A potential alternative to conventional antibiotic therapy
- Author Details:
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Oviya Priya I
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Priyadarshini Shanmugam
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Ankush Chauhan
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Alice Peace Selvabai *
Oviya Priya I
Priyadarshini Shanmugam
Alice Peace Selvabai *
Background: Antimicrobial resistance (AMR) poses a global threat and reported as a leading cause for morbidity and mortality. It is mediated by several factors like irrational use of antibiotics for treatment in various diseases, the misuse of antibiotics, especially in livestocks, improper hygiene and infection control practices and environmental contaminants. As a result, the efficacy of antibiotics has been inhibited and there is an urgent need for newer antibiotics. Therefore our study is aimed to explore the anti-bacterial activity of herbal extracts which would act as an alternate herbal therapy to fight against the multidrug-resistant (MDR) pathogens.
Materials and Methods: The multidrug resistant (MDR) bacterial isolates were obtained from various clinical specimens from wound swab, urine, pus aspirates, blood, endotracheal aspirates, and sputum samples according to standard microbiological protocols. In our study 15 herbs were selected for methanolic extraction by standard Soxhlet extraction method. The extracts were then tested against the multi-drug resistant pathogens by well diffusion method and interpreted.
Results: The resistant bacterial strains such as Methicillin-Resistant Staphylococcus aureus (MRSA), Escherichia coli, Proteus species, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter species were used as the test strains in the study. Remarkably 7 Out of the 15 herbal extracts tested for anti-bacterial activity showed susceptibility to the resistant strains. Among the 7 effective herbal extracts Juglans regia had effectiveness even in ethanol.
About 60% of the MRSA strains were susceptible to several herbal extracts which includes Phyllanthus edulis, Phyllanthus debilis, Juglans regia, Justicia adathoda, Ormocarpum cochinchinense, Mentha and Andrographis alata. Proteus species showed susceptibility to Phyllanthus edulis, Phyllanthus debilis, Justicia adathoda. Acinetobacter species showed susceptibility to Phyllanthus edulis, Mentha and Justicia adathoda. Pseudomonas aeruginosa strains were susceptible to Phyllanthus edulis and Justicia adathoda.
Conclusion: The preliminary findings of this study is that certain herbal extracts exhibit antibacterial activity against multidrug-resistant pathogens. And so that such extracts may have potential as natural therapeutic agents. Further studies are required to isolate the active phytochemicals and their clinical applicability.
References
- No: IHEC-II/0386/23.
- Author Contributions This research work was carried out through the collective effort of all authors. All authors contributed equally to the study and manuscript preparation and have approved the final version for publication.
- Acknowledgements The authors would like to express sincere gratitude to Chettinad Academy of Research and Education (CARE), Chettinad Hospital and Research Institute (CHRI), Department of Microbiology for providing the necessary facilities and support to carry out this research and for their assistance during the study. References
- World Health Organization. Antimicrobial resistance: Report by the Director-General. Seventy-seventh World Health Assembly [Internet]. Geneva: WHO; 2024. Report No.: A77/5. Available from: https://apps.who.int/gb/ebwha/pdf_files/WHA77/A77_5- en.pdf.
- Mohanapriya TD, Mohankumar A, Selvabai AP, Jayaraman P, Shanmugam P. Exploring multidrug resistance patterns in Escherichia coli isolated from the gut of healthy individuals: a comprehensive analysis. J Commun Dis. 2023;55(3):21–7.
- GBD 2021 Antimicrobial Resistance Collaborators. Global burden of bacterial antimicrobial resistance 1990–2021: a systematic analysis with forecasts to 2050. Lancet. 2024;404(10459):1199–
- Zouine N, El Ghachtouli N, El Abed S, Koraichi SI. A comprehensive review on medicinal plant extracts as antibacterial agents: factors, mechanism insights and future prospects. Sci Afr. 2024;26:e02395.
- Kebede T, Gadisa E, Tufa A. Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: a possible alternative in the treatment of multidrug-resistant microbes. PLoS One. 2021;16(3):e0249253.
- Mahady GB. Medicinal plants for the prevention and treatment of bacterial infections. Curr Pharm Des. 2005;11(19):2405–27.
- Ahmed ET, Dukessa A, Mateos T, Meka W, Seyoum MM, Zawdie B. Evaluation of the antidyslipidemic and nephroprotective effect of methanolic seed extract of Lepidium sativum on male Swiss albino mice fed on deep fried palm oil. Front Nutr. 2025;12:1468704.
- Bernaitis L, Shobha KL, Ashok M, Shenoy RP, Mathew JJ, Khan DM. Comparative evaluation of the antimicrobial activity of ethanol extract of Taxus baccata, Phyllanthus debilis, Plectranthus amboinicus against multi drug resistant bacteria. Int J Pharm Sci Res. 2013;4(8):3147–50.
- Teow SY, Liew K, Ali SA, Khoo AS, Peh SC. Antibacterial action of curcumin against Staphylococcus aureus: a brief review. J Trop Med. 2016;2016:2853045. 328 Priya I et al. / Indian Journal of Microbiology Research 2025;12(3):323–328
- Yadeta NC. Phytochemical investigation and antioxidant activity of root extract of Aloe gilbertii Reynolds, from Konso, Southern Ethiopia. Am J Appl Chem. 2019;7(5):130–6.
- Muqbil I, Masood A, Sarkar FH, Mohammad RM, Azmi AS. Progress in nanotechnology-based approaches to enhance the potential of chemopreventive agents. Cancers. 2011;3(1):428–45.
- Mitra S, Bhesania Hodiwala AV, Kar H. Susceptibility and synergistic effects of guava plant extract and antimicrobial drugs on Escherichia coli. Cureus. 2024;16(1):e52345.
- Nimri LF, Meqdam MM, Alkofahi A. Antibacterial activity of Jordanian medicinal plants. Pharm Biol. 1999;37(3):196–201.
- Behzad F, Naghib SM, Kouhbanani MAJ, Tabatabaei SN, Zare Y, Rhee KY. An overview of the plant-mediated green synthesis of noble metal nanoparticles for antibacterial applications. J Ind Eng Chem. 2020;94:92–104.
- Jadoun S., Arif R., Jangid NK, Meena RK. Green synthesis of nanoparticles using plant extracts: a review. Environ Chem Lett. 2021;19:355–74. Cite this article: Priya IO, Shanmugam P, Chauhan A, Selvabai AP. Efficacy of plant-derived extracts against multidrug resistant pathogens: a potential alternative to conventional antibiotic therapy. Indian J Microbiol Res. 2025;12(3):323–328.