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- DOI 10.18231/j.ijmr.18950.1758359451
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CrossMark
Evaluation of polyester polyurethane-degrading capability of bacteria isolated from landfill sites
- Author Details:
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Xuan Ngoc Thanh Nguyen
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Nguyen Nu Thao Lu
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Tham Van Hong Vo
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Hai Ngoc Nguyen
Xuan Ngoc Thanh Nguyen
Background: Polyurethane (PU) is one of the most widely used categories of plastics in modern life. The enormous demand for PU has contributed to the global plastic crisis. In recent years, microbial strains capable of degrading plastics have garnered significant scientific interest. In the study, bacterial strains were isolated from natural environment, such as soil and waste plastics, and screened for PU-degrading activity using Impranil as a model substrate.
Materials and Methods: The PU-degrading activity was evaluated on both liquid (Impranil) and solid (PU foam) substrates. Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM) analyses were used to detect chemical and physical changes.
Results: FTIR spectra reveal that the urethane, and ester components, along with carbon chains in Impranil and PU foam, were degraded by the microorganisms. On the liquid substrate (Impranil), degradation was observed in urethane, and ester bonds. On the solid substrate (PU foam), it is likely that the urethane component was attacked by the bacterial strain. SEM images disclosed that fiber density of PU foam in inoculated nutrient broth (NB) ppeared to be lower compared to the control. The PU-degrading strains were identified as Bacillus velezensis.
Conclusion: These findings demonstrate that microorganisms from natural environment could play a significant role in addressing the global plastic pollution.
References
- Chalmin P. The history of plastics: from the Capitol to the Tarpeian Rock. Field Actions Sci Rep. 2019;(Spec Issue 19):6–11. 370 Nguyen et al. / Indian Journal of Microbiology Research 2025;12(3):364–371
- Crystal Thew XE, Lo SC, Ramanan RN, Tey BT, Nguyen DH, Wei OC. Enhancing plastic biodegradation process: strategies and opportunities. Crit Rev Biotechnol. 2023;44(3):477–94.
- Kumar L. A review on biodegradation of post-production and post- consumer polyurethane wastes. Int J Adv Multidiscip Res. 2002;9(5):110–25.
- Ritchie H. How much of global greenhouse gas emissions come from plastics [Internet]. OurWorldinData.org; 2023. Available from: https://ourworldindata.org/ghg-emissions-plastics.
- Brunner I, Fischer M, Rüthi J, Stierli B, Frey B. Ability of fungi isolated from plastic debris floating in the shoreline of a lake to degrade plastics. PLoS One. 2018;13(8):e0202047.
- Gui Z, Liu G, Liu X, Cai R, Liu R, Sun C. A deep-sea bacterium is capable of degrading Polyurethane. Microbiol Spectr. 2023;11(3):e0007323.
- Howard GT, Norton WN, Burks T. Growth of Acinetobacter gerneri P7 on polyurethane and the purification and characterization of a polyurethanase enzyme. Biodegradation. 2021; 23(4):561–73.
- Kim JH, Choi SH, Park MG, Park DH, Son KH, Park HY. Polyurethane biodegradation by Serratia sp. HY-72 isolated from the intestine of the Asian mantis Hierodula patellifera. Front Microbiol. 2022;13:1005415.
- Mukherjee K, Tribedi P, Chowdhury A, Ray T, Joardar A, Giri S, et al. Isolation of a Pseudomonas aeruginosa strain from soil that can degrade polyurethane diol. Biodegradation. 2011;22(2):377–88.
- Oceguera-Cervantes A, Carrillo-García A, López N, Bolaños-Nuñez S, Cruz-Gómez MJ, Wacher C, et al. Characterization of the polyurethanolytic activity of two Alicycliphilus sp. strains able to degrade polyurethane and N-methylpyrrolidone. Appl Environ Microbiol. 2007;73(19):6214–23.
- Orts JM, Parrado J, Pascual JA, Orts A, Cuartero J, Tejada M, et al. Polyurethane foam residue biodegradation through the Tenebrio molitor digestive tract: microbial communities and enzymatic activity. Polymers. 2023;15(1):204.
- Salgado CA, Silva JG, Almeida FA, Vanetti MCD. Biodegradation of polyurethanes by Serratia liquefaciens L135 and its polyurethanase: in silico and in vitro analyses. Environ Pollut. 2023;333:122016.
- Kemona A, Piotrowska M. Polyurethane recycling and disposal: methods and prospects. Polymers. 2020;12(8):1752.
- de Souza FM, Kahol PK, Gupta RK. Introduction to polyurethane chemistry. In: Polyurethane chemistry: Renewable polyols and isocyanates. American Chemical Society; 2021. pp. 1-24.
- Maestri C, Plancher L, Duthoit A, Hébert RL, Di Martino P. Fungal biodegradation of polyurethanes. J Fungi. 2023;9(7):760.
- Rajan A, Ameen F, Jambulingam R, Shankar V. Biodegradation of polyurethane by fungi isolated from industrial wastewater—a sustainable approach to plastic waste management. Polymers. 2024;16(10):1411.
- Russell JR, Huang J, Anand P, Kucera K, Sandoval AG, Dantzler KW, et al. Biodegradation of polyester polyurethane by endophytic fungi. Appl Environ Microbiol. 2011;77(17):6076–84.
- Wu KY, Yang TX, Yang M, Wu JQ, Li X, Chen XD, et al. Preliminary identification of soil fungi for the degradation of polyurethane film. Arch Microbiol. 2023;205(4):145.
- Peng YH, Shih YH, Lai YC, Liu YZ, Liu YT, Lin NC. Degradation of polyurethane by bacterium isolated from soil and assessment of polyurethanolytic activity of a Pseudomonas putida strain. Environ Sci Pollut Res Int. 2014;21(16):9529–37.
- Schmidt J, Wei R, Oeser T, Dedavid E Silva LA, Breite D, Schulze A, et al. Degradation of polyester polyurethane by bacterial polyester hydrolases. Polymers (Basel). 2017;9(2):65.
- Shah Z, Krumholz L, Aktas DF, Hasan F, Khattak M, Shah AA. Degradation of polyester polyurethane by a newly isolated soil bacterium, Bacillus subtilis strain MZA-75. Biodegradation. 2013;24(6):865–77.
- Rowe L, Howard GT. Growth of Bacillus subtilis on polyurethane and the purification and characterization of a polyurethanase-lipase enzyme. Int Biodeterior Biodegradation. 2002;50(1):33–40.
- Yazhini VS, Prabha ML, Issac R. Characterization and molecular identification of poly urethane degrading bacteria. J Pure Appl Microbiol. 2021;15(3):1291–300.
- Zarezadeh E, Tangestani M, Jafari AJ. A systematic review of methodologies and solution for recycling polyurethane foams to safeguard environment. Heliyon. 2024;10:e40724.
- Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, et al. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol. 1998;64(2):795–9.
- Biffinger JC, Barlow DE, Pirlo RK, Babson DM, Fitzgerald LA, Zingarelli S, et al. A direct quantitative agar-plate based assay for analysis of Pseudomonas protegens Pf-5 degradation of polyurethane films. Int Biodeterior Biodegradation. 2014;95(Pt B):311–9.
- Liu J, He J, Xue R, Xu B, Qian X, Xin F, et al. Biodegradation and up-cycling of polyurethanes: progress, challenges, and prospects. Biotechnol Adv. 2021;48:107730.
- Dulyanska Y, Cruz-Lopes L, Esteves B, Guiné R, Domingos I. FTIR monitoring of polyurethane foams derived from acid-liquefied and base-liquefied polyols. Polymers. 2024;16(15):2214.
- Francés AB, Banon MVN. Effect of silica nanoparticles on polyurethane foaming process and foam properties. In: IOP Conference Series: Materials Science and Engineering. Bristol, UK: IOP Publishing; 2014. p. 012020.
- Smith BC. Infrared spectroscopy of polymers XIII: polyurethanes. Spectroscopy. 2023;38(7):14–6.
- Mora-Murillo LD, Orozco-Gutierrez F, Vega-Baudrit J, González- Paz RJ. Thermal-mechanical characterization of polyurethane rigid foams: effect of modifying bio-polyol content in isocyanate prepolymers. J Renew Mater. 2017;5(3-4):220–30.
- Paberza A, Fridrihsone-Girone A, Abolins A, Cabulis U. Polyols from recycled poly(ethylene terephthalate) flakes and rapeseed oil for polyurethane foams. Polimery. 2015;60(9):572–8.
- Plancher L, Nguyen GTM, Hébert R, Maestri C, Mélinge Y, Ledésert B, et al. Oxidative biodegradation of a solid-solid polyether-urethane phase change material by Penicillium and Aspergillus. Mater Today Commun. 2021;29:102949.
- Beneš H, Černá R, Ďuračková A, Petra L. Utilization of natural oils for decomposition of polyurethanes. J Polym Environ. 2012;20(1):175–85.
- Wärnheim A, Kotov N, Dobryden I, Leggieri RT, Edvinsson C, Heydari G, et al. Nanomechanical and nano-FTIR analysis of polyester coil coatings before and after artificial weathering experiments. Prog Org Coat. 2024;190:108355.
- Neswati, Nazir N, Arief S, Yusniwati. Improvement of flexible polyurethane foam characteristics of palm oil polyols with the addition of polyethylene glycol 400. In: IOP Conference Series: Earth and Environmental Science. Bristol, UK: IOP Publishing;
- p. 012031.
- Członka S, Strąkowska A, Kairytė A. Application of walnut shells- derived biopolyol in the synthesis of rigid polyurethane foams. Materials (Basel). 2020;13(12):2687.
- Su T, Zhang T, Liu P, Bian J, Zheng Y, Yuan Y, et al. Biodegradation of polyurethane by the microbial consortia enriched from landfill. Appl Microbiol Biotechnol. 2023;107(5-6):1983–95.
- Ruiz-García C, Béjar V, Martínez-Checa F, Llamas I, Quesada E. Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Vélez in Málaga, southern Spain. Int J Syst Evol Microbiol. 2005;55(Pt 1):191–5.
- Byun H, Brockett MR, Pu Q, Hrycko AJ, Beld J, Zhu J. An intestinal Bacillus velezensis isolate displays broad-spectrum antibacterial activity and prevents infection of both Gram-Positive and Gram- Negative pathogens in vivo. J Bacteriol. 2023;205(6):e0013323.
- Liu H, Jiang J, An M, Li B, Xie Y, Xu C, et al. Bacillus velezensis SYL-3 suppresses Alternaria alternata and tobacco mosaic virus infecting Nicotiana tabacum by regulating the phyllosphere microbial community. Front Microbiol. 2022;13:840318. Nguyen et al. / Indian Journal of Microbiology Research 2025;12(3):364–371 371
- Su T, Shen B, Hu X, Teng Y, Weng P, Wu Z, et al. Research advance of Bacillus velezensis: bioinformatics, characteristics, and applications. Food Sci Hum Wellness. 2024;13(4):1756–66.
- Li Y, Chen S, Yu Z, Yao J, Jia Y, Liao C, et al. A novel Bacillus velezensis for efficient degradation of zearalenone. Foods. 2024;13(4):530.
- Zeng J, Yao J, Zhang W, Zhang M, Wang T, Yu X, et al. Biodegradation of commercial polyester polyurethane by a soil- borne bacterium Bacillus velezensis MB01B: efficiency, degradation pathway, and in-situ remediation in landfill soil. Environ Pollut. 2024;363(Pt 2):125300.
- Liu J, Zeng Q, Lei H, Xin K, Xu A, Wei R, et al. Biodegradation of polyester polyurethane by Cladosporium sp. P7: evaluating its degradation capacity and metabolic pathways. J Hazard Mater. 2023;448:130776.
- Nakkabi A, Moulay Sadiki M, Fahim M, Najim Ittobane N, IbnsoudaKoraichi S, Barkai H, et al. Biodegradation of poly(ester urethane)s by Bacillus subtilis. Int J Environ Res. 2015;9(1):157–
- Magami S, Akimoto H. The degradation mechanism of high- strength polyurethane elastomers in the presence of water. Komatsu Tech Rep. 2023;69(176):26–33.
- Singhal P, Small W, Cosgriff-Hernandez E, Maitland DJ, Wilson TS. Low density biodegradable shape memory polyurethane foams for embolic biomedical applications. Acta Biomater. 2014;10(1):67–76.
- Álvarez-Barragán J, Domínguez-Malfavón L, Vargas-Suárez M, González-Hernández R, Aguilar-Osorio G, Loza-Tavera H. Biodegradative activities of selected environmental fungi on a polyester polyurethane varnish and polyether polyurethane foams. Appl Environ Microbiol. 2016;82(17):5225–35.
- De Smet D, Verjans J, Bader M, Mondschein A, Vanneste M. Design of biodegradable PU textile coating. Polymers. 2024;16(16):2236.
- Nakkabi A, Ittobane N, Dary M, Duran EM, Fahim M. Growth of Pseudomonas in polyurethane medium. IOSR J Eng. 2015;5(7):36–
- Nakkabi A, Sadiki M, Ibnssouda S, Fahim M. Biological degradation of polyurethane by a newly isolated wood bacterium. Int J Recent Adv Multidiscip Res. 2015;2(2):222–5.
- Oprea S. Dependence of fungal biodegradation of PEG/castor oil- based polyurethane elastomers on the hard-segment structure. Polym Degrad Stab. 2010;95(12):2396–404.
- Frautschi JR, Chinn JA, Phillips RE, Zhao QH, Anderson JM, Joshi R, et al. Degradation of polyurethanes in vitro and in vivo: comparison of different models. Colloids Surf B Biointerfaces. 1993;1(5):305–13.
- Sarkar D, Lopina ST. Oxidative and enzymatic degradations of l- tyrosine based polyurethanes. Polym Degrad Stab . 2007;92(11):1994–2004.
- Fuentes-Jaime J, Vargas-Suárez M, Cruz-Gómez MJ, Loza-Tavera H. Concerted action of extracellular and cytoplasmic esterase and urethane-cleaving activities during Impranil biodegradation by Alicycliphilus denitrificans BQ1. Biodegradation. 2022;33(4):389–
- Magaña-Montiel N, Muriel-Millán LF, Pardo-López L. XTT assay for detection of bacterial metabolic activity in water-based polyester polyurethane. PLoS One. 2024;19(6):e0303210.
- Park WJ, Hwangbo M, Chu KH. Plastisphere and microorganisms involved in polyurethane biodegradation. Sci Total Environ. 2023;886:163932.
- Cai Z, Li M, Zhu Z, Wang X, Huang Y, Li T, et al. Biological degradation of plastics and microplastics: a recent perspective on associated mechanisms and influencing factors. Microorganisms. 2023;11(7):1661.
- Magnin A, Pollet E, Phalip V, Avérous L. Evaluation of biological degradation of polyurethanes. Biotechnol Adv. 2020;39:107457.
- Magnin A, Pollet E, Perrin R, Ullmann C, Persillon C, Phalip V, et al. Enzymatic recycling of thermoplastic polyurethanes: synergistic effect of an esterase and an amidase and recovery of building blocks. Waste Manag. 2019;85:141–50.